NanoPlot report
Summary statistics
| feature |
|
| General summary |
|
| Average percent identity |
88.4 |
| Mean read length |
717.5 |
| Mean read quality |
9.8 |
| Median percent identity |
88.9 |
| Median read length |
570.0 |
| Median read quality |
10.0 |
| Number of reads |
2,705.0 |
| Read length N50 |
1,069.0 |
| Total bases |
1,940,768.0 |
| Total bases aligned |
1,800,180.0 |
| Number, percentage and megabases of reads above quality cutoffs |
|
| >Q5 |
2705 (100.0%) 1.9Mb |
| >Q7 |
2590 (95.7%) 1.9Mb |
| >Q10 |
1339 (49.5%) 1.2Mb |
| >Q12 |
157 (5.8%) 0.1Mb |
| >Q15 |
1 (0.0%) 0.0Mb |
| Top 5 highest mean basecall quality scores and their read lengths |
|
| 1 |
17.0 (194; 8304fa77-b338-40dc-92fc-7fe1267ea322) |
| 2 |
14.0 (1065; 5aa6fea6-ed62-4f1b-a45e-5cd08af36cc3) |
| 3 |
13.9 (107; cd401ce5-fa9d-40b4-8bb8-4526ec3316f1) |
| 4 |
13.6 (1097; f0696aab-f5c4-4375-bbad-e42981df53d3) |
| 5 |
13.6 (113; 15d399e1-bff1-4451-9561-5d6035386fdc) |
| Top 5 longest reads and their mean basecall quality score |
|
| 1 |
3019 (10.7; 3c2580da-ce9f-4997-8ee4-8ab2341d5cc7) |
| 2 |
2988 (11.5; 860e9c82-380a-4e02-968a-7a274972d1ac) |
| 3 |
2979 (12.2; 6a287cc1-3c00-4ab9-8897-12a0ce685c18) |
| 4 |
2941 (11.9; ec3e1462-4aa5-4d76-a098-d501e3c255d6) |
| 5 |
2887 (12.4; 7f89e506-3d0e-4090-8a50-3547221831ab) |
Plots
Histogram of read lengths
Weighted Histogram of read lengths
sub3_full.sorted.bam
Yield by length
Read lengths vs Average read quality plot using hexagonal bins
Read lengths vs Average read quality plot using dots
Read lengths vs Average read quality plot using a kernel density estimation
Aligned read lengths vs Sequenced read length plot using hexagonal bins
Aligned read lengths vs Sequenced read length plot using dots
Aligned read lengths vs Sequenced read length plot using a kernel density estimation
Read mapping quality vs Average basecall quality plot using hexagonal bins
Read mapping quality vs Average basecall quality plot using dots
Read mapping quality vs Average basecall quality plot using a kernel density estimation
Read length vs Read mapping quality plot using hexagonal bins
Read length vs Read mapping quality plot using dots
Read length vs Read mapping quality plot using a kernel density estimation
Percent identity vs Average Base Quality plot using hexagonal bins
Percent identity vs Average Base Quality plot using dots
Percent identity vs Average Base Quality plot using a kernel density estimation
Aligned read length vs Percent identity plot using hexagonal bins
Aligned read length vs Percent identity plot using dots
Aligned read length vs Percent identity plot using a kernel density estimation
sub3_full.sorted.bam